GRIVAS

PRINCIPAL INVESTIGATORS:

  • Enrique Guzmán-Gutiérrez.

E-Mail:

  • eguzman@udec.cl

INSTITUTION:
Universidad de Concepción

COUNTRY:
Chile

CITY:
Concepción

LABORATORY NAME:
Pregnancy diseases Laboratory.

Research lines

  • Main focus of my laboratory is understanding the role of thyroid hormones in pregnancies diseases, in particular gestational diabetes mellitus.
  • Our scientific production has used maternal plasma blood obtained during pregnancy, human placenta (trophoblast culture) and fetal plasma blood.

Major equipment available

  • Cell culture room full equipped; Microscopy (inverse, fluorescent); Equipment for cellular and molecular biology (PCR, Western blot, immunocytochemistry).

Techniques available

  • Available techniques are isolation and primary culture of HUVEC and trophoblast Biological techniques include RT-PCR, qPCR, Western  blot, siRNA, etc.

General interest of collaboration

  • Fully open to collaborate in vascular placental physiology and pregnancy pathologies.

Financial support

  • FONDECYT initiation investigation 11170710 (2017-2020)
    Principal Investigator (2017-11 / 2020-10). Maternal Thyroid Profile characterization during pregnancy: Association with gestational diabetes, altered thyroid metabolism in placenta and neonatal thyroid profile.
  • INTERNATIONAL NETWORKS REDI170287 (2018-2019)
    Principal Investigator (2018-01 / 2019-12). Strengthening of the cooperation network for the study of vascular disorders associated with pregnancy disorders in Latin America. University of Concepción.
  • CORFO 17CTEBI-83482 (2018) Principal Investigator (2018-01 / 2018-12). Expression of isoforms of the insulin receptor and its association with canine mammary neoplasms in the Biobio region. San Sebastián University.
  • FONDEF ID16I10221 (2017-2018) Researcher (2017-01 / 2018-07). Development of Optoelectronic Biosensors and Correlation Algorithms to quantify the spectrum emitted by gold nanoparticles that detect the presence of residues of antibiotics in raw milk. San Sebastián University. Director: Dr. Sergio Torres, University of Concepción.
  • DIUSS 2016-0011-GI (2017-2018) Principal Investigator (2017-03 / 2018-12). Effect of Cotinine on endothelial dysfunction observed in pregnancies with preeclampsia. San Sebastian University.
  • CORFO 2016-67535 (2017) Principal Investigator (2017-01 / 2017-08). Detection of miRNA as a predictor of gestational diabetes in the commune of Hualqui, Biobio Region. San Sebastián University.
  • CONICYT-Regional VCE-40000005 (2015-2016)
    Researcher (2015-03 / 2016-02). Development of a model of scientific linkage with the agro-alimentary business sector cattleman of the Biobío to address the animal health problem through the implementation of innovations and biotechnological solutions. San Sebastián University.
  • CONICYT-Thesis Support 24120945 (2012-2014)
    Principal Investigator (2012-08 / 2014-07). Regulation of L-arginine transport by insulin via hCAT-1 involves differential activation of subtypes A and B insulin receptors and adenosine receptors in HUVEC. Pontifical Catholic University of Chile.

Publication list

  1. Alcala M, Gutierrez-Vega S, Castro E, Guzmán-Gutiérrez E, Ramos-Alvarez MP, Viana M. Antioxidants and oxidative stress: focus in obese pregnancies. Frontiers in Physiology. 2018 (En prensa).
  2. Contreras-Trigo B, Díaz-García V, Guzmán-Gutierrez E, Sanhueza I, Coelho P, Godoy SE, Torres S, Oyarzún P. Slight pH Fluctuations in the Gold Nanoparticle Synthesis Process Influence the Performance of the Citrate Reduction Method. Sensors 2018; 12;18(7). doi: 3390/s18072246.
  3. Troncoso F, Acurio J, Herlitz K, Aguayo C, Bertoglia P, Guzman-Gutierrez E, Loyola M, Gonzalez M, Rezgaoui M, Desoye G, Escudero C. Gestational diabetes mellitus is associated with increased pro-migratory activation of vascular endothelial growth factor receptor 2 and reduced expression of vascular endothelial growth factor receptor 1. Plos One. 2017;12(8):e0182509. doi: 10.1371/journal.pone.0182509
  4. Leiva A, Guzmán-Gutiérrez E, Contreras-Duarte S, Fuenzalida B, Cantin C, Carvajal L, Salsoso R, Gutiérrez J, Pardo F, Sobrevia L. Adenosine receptors: Modulators of lipid availability that are controlled by lipid levels. Mol Aspects Med. 2017 doi: 10.1016/j.mam.2017.01.007.
  5. Guzmán-Gutiérrez E, Armella A, Toledo F, Pardo F, Leiva A, Sobrevia L. Insulin requires A1 adenosine receptors expression to reverse gestational diabetes-increased L-arginine transport in human umbilical vein endothelium. Purinergic Signal. 2015;12(1):175-90. doi: 10.1007/s11302-015-9491-2
  6. Herlitz-Cifuentes HS, Garces PC, Fernandez LI, Guzman-Gutierrez EA. Effect of Systemic Inflammation on the Function of Insulin and Glucose Metabolism in Rheumatoid Arthritis. Curr Diabetes Rev. 2015; 12(2):156-62.
  7. González M, Rojas S, Avila P, Cabrera L, Villalobos R, Palma C, Aguayo C, Peña E, Gallardo V, Guzmán-Gutiérrez E, Sáez T, Salsoso R, Sanhueza C, Pardo F, Leiva A, Sobrevia L. Insulin reverses D-glucose-increased nitric oxide and reactive oxygen species generation in human umbilical vein endothelial cells. PLoS One. 2015; 10(4):e0122398. doi: 10.1371/journal.pone.0122398.
  8. Salsoso R, Guzmán-Gutiérrez E, Sáez T, Bugueño K, Ramírez MA, Farías M, Pardo F, Leiva A, Sanhueza C, Mate A, Vázquez C, Sobrevia L. Insulin restores L-arginine transport requiring adenosine receptors activation in umbilical vein endothelium from late-onset preeclampsia. Placenta 2015; 36:287-296.
  9. Westermeier F, Salomón C, Farías M, Arroyo P, Fuenzalida B, Sáez T, Salsoso R, Sanhueza C, Guzmán-Gutiérrez E, Pardo F, Leiva A, Sobrevia L. Insulin requires normal expression and signaling of insulin receptor A to reverse gestational diabetes-reduced adenosine transport in human umbilical vein endothelium. FASEB J 2014; 29:37-49.
  10. Guzmán-Gutiérrez E, Veas C, Leiva A, Escudero CA, Sobrevia L. Is a low level of free thyroxine in the maternal circulation associated with altered endothelial function in Gestational Diabetes? Frontiers in Pharmacology 2014; 5:136. doi: 10.3389/fphar.2014.00136.
  11. Guzmán-Gutiérrez E, Arroyo P, Salsoso R, Fuenzalida B, Sáez T, Leiva A, Pardo F, Sobrevia L. Role of Insulin and Adenosine in the Human Placenta Microvascular and Macrovascular Endothelial Cell Dysfunction in Gestational Diabetes Mellitus. Microcirculation 2014; 21:26-37.
  12. Salsoso R, Guzmán-Gutiérrez E, Arroyo P, Salomón C, Zambrano S, Ruiz-Armenta MV, Blanca AJ, Pardo F, Leiva A, Mate A, Sobrevia L, Vázquez CM. Reduced L-carnitine transport in aortic endothelial cells from spontaneously hypertensive rats. PLoS One 2014;9:e90339.
  13. Arroyo P, Guzmán-Gutiérrez E, Pardo F, Salomón C, Westermeier F, Salsoso R, Sáez T, Leiva A, Sobrevia L. Gestational Diabetes Mellitus and the Role of Adenosine in the Human Placental Endothelium and Central Nervous System. GJPM 2013; 1:24-42.
  14. Pardo F, Arroyo P, Salomón C, Westermeier F, Salsoso R, Sáez T, Guzmán-Gutiérrez E, Leiva A, Sobrevia L. Role of Equilibrative Adenosine Transporters and Adenosine Receptors as Modulators of the Human Placental Endothelium in Gestational Diabetes Mellitus. Placenta 2013; 34:1121-1127. doi: 10.1016/j.placenta.2013.09.007.
  15. Leiva A, Diez de Medina C, Sáez T, San Martín S, Abarzúa F, Farías M, Guzmán-Gutiérrez E, Pardo F, Sobrevia L. Maternal hypercholesterolemia in pregnancy associates with placenta endotelial dysfunction: role of eNOS and arginase II. Arterioscler Thromb Vasc Biol 2013; 33:2444-2453.
  16. Aravena C, Beltrán AR, Cornejo M, Torres V, Díaz ES, Guzmán-Gutiérrez E, Pardo F, Leiva A, Sobrevia L, Ramírez MA. Potential Role of Sodium-Proton Exchangers in the Low Concentration Arsenic Trioxide-Increased Intracellular pH and Cell Proliferation. PLoS One 2012; 7:e51451.
  17. Guzmán-Gutiérrez E, Westermeier F, Salomón C, González M, Pardo F, Leiva A, Sobrevia L. Insulin-Increased L-Arginine Transport Requires A(2A) Adenosine Receptors Activation in Human Umbilical Vein Endothelium. PLoS One 2012; 7:e41705.
  18. Salomón C, Westermeier F, Puebla C, Arroyo P, Guzmán-Gutiérrez E, Pardo F, Leiva A, Casanello P, Sobrevia L. Gestational diabetes reduces adenosine transport in human placental microvascular endothelium, an effect reversed by insulin. PLoS One 2012; 7:e40578.
  19. Guzmán-Gutiérrez E, Abarzúa F, Belmar C, Nien JK, Ramírez MA, Arroyo P, Salomón C, Westermeier F, Puebla C, Leiva A, Casanello P, Sobrevia L. Functional link between adenosine and insulin: a hypothesis for fetoplacental vascular endothelial dysfunction in gestational diabetes. Curr Vasc Pharmacol 2011; 9:750-762.
  20. Westermeier F, Salomón C, González M, Puebla C, Guzmán-Gutiérrez E, Cifuentes F, Leiva A, Casanello P, Sobrevia L. Insulin restores restational diabetes mellitus-reduced adenosine transport involving differential expression of insulin receptor isoforms in human umbilical vein endothelium. Diabetes 2011; 60:1677-87.
  21. González M, Gallardo V, Rodríguez N, Salomón C, Westermeier F, Guzmán-Gutiérrez E, Leiva A, Abarzúa F, Casanello P, Sobrevia L. Insulin-stimulated L-arginine transport requires SLC7A1 gene expression and is associated with human umbilical vein relaxation. J Cell Physiol 2011; 226:2916-24.
  22. Sobrevia L, Abarzúa F, Nien JK, Salomón C, Westermeier F, Puebla C, Cifuentes F, Guzmán-Gutiérrez E, Leiva A, Casanello P. Review: Differential placental macrovascular and microvascular endothelial dysfunction in gestational diabetes. Placenta 2011; 32:S159-S164.
  23. Guzmán-Gutiérrez E, Sandoval C, Nova E, Castillo JL, Vera JC, Lamperti L, Krause B, Salomon C, Sepulveda C, Aguayo C, Sobrevia L. Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells. Placenta 2010, 31:928-936.