Laboratorio de Fisiopatología Placentaria

• Mariana Farina (Researcher)
• Tomás Etcheverry (Doctoral student)
• Gustavo Leguizamón (MSc in Medical Molecular Biology)

• Role of aquaporins in extravillous and villous trophoblast, endothelial cells and fetal membranes of human normal and pathological placentas.
• Serum uric acid in normal and preeclamptic pregnancies.
• Role of endocannabinoid system in normal and preeclamptic placentas.
• Mechanisms underlying the anomalies of growth and development in embryos, fetuses and placentas and the programming of metabolic impairments in the offspring of Diabetic mother with emphasis in the role of nutrient sensors like mammalian target of Rapamicin (mTOR) and peroxisome proliferator activated receptors (PPARs).
• Mechanisms underlying the metabolic impairments in placentas, fetuses and offspring from obese mothers.
• Role of FOXO1 in the development of cardiopathy in the embryos and offspring from diabetic mothers.

• Hypoxic Chamber for cell and explants culture
• Epifluorescence Microscopy (Nikon Eclipse E200, Nikon Instruments Inc).
• Using modified chamber water flux determinations in cell cultures and fetal membranes
• Real Time qPCR (Rotorgene 6000).
• Cryostat

• qRT-PCR
• Western blot
• Radioimmunoassay
• NOS activity
• Immunofluorescence and Immunohistochemistry techniques
• Culture of placental explants and human fetal membranes
• Cellular lines: Swan71, HTR8/Svneo, JEG-3, BeWo, EA.hy926, HT-1080
• Apoptosis parameters
• Oxidative stress determinations
• Wound healing (migration assay)
• Zymography
• Invasion assay (in Matrigel)
• Tube like-formation assay
• Membrane lipid quantification
• Water flux measures in Ussing modified chambers
• Thin layer chromatography
• Nitrative stress determinations
• Superoxide Dismutase activity

• Placentas and blood samples exchange
• Exchange of medical Records from normal and pathological pregnancies
• Skill collaboration

“Rol de la acuaporina-9 placentaria en el desencadenamiento de la preeclampsia”

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: January 2016- December 2018

“Participación de los microdominios de membrana en la regulación del sistema endocannabinoide en placentas normales y preeclámpticas. Implicancias en la apoptosis del trofoblasto”.

• Source: CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS
• Duration: January 2017- December 2019

 “Implicancias de la Hipoxia/Reoxigenación en el proceso de sincialización. Rol del sistema endocannabinoide y su asociación a microdominios de membrana”.

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: January 2017- December 2019

“Fallas en mecanismos de regulación nutricional condicionan el éxito reproductivo y determinan el desarrollo de la placenta en forma transgeneracional en patologías metabólicas en la gestación”.

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: December 2015- December 2018

“Análisis de la función feto-placentaria de los receptores nucleares PPAR en un novel modelo de diabetes gestacional inducida por programación intrauterina”.

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: January 2017- January 2020

“Patologías metabólicas en la gestación: Suplementación con ácidos grasos insaturados como reguladores de vías de los PPAR y de mTOR en diferentes etapas de la preñez”

• Source: CONSEJO NACIONAL DE INVESTIGACIONES CIENTÍFICAS Y TÉCNICAS
• Duration: January 2017- January 2020

“Influencia de la sobrecarga de grasas saturadas en la dieta materna sobre la programación de la función de detoxificación en el hígado e intestino en la descendencia de rata: modulación por butirato”

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: January 2017- January 2020

 “Efecto de la adición de ácidos grasos insaturados en la dieta de mujeres embarazadas sanas y con diabetes sobre marcadores del entorno pro-oxidante y pro-inflamatorio en la placenta y sangre materna y de cordón fetal”.

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: June 2017- June 2020

“Embriopatía diabética y programación intrauterina de alteraciones inducidas por la diabetes materna: Rol del factor de transcripión FOXO1”

• Source: AGENCIA NACIONAL DE PROMOCIÓN CIENTÍFICA Y TECNOLÓGICA
• Duration: January 2017- January 2020

1. Damiano AE, Zotta E, Goldstein J, Reisin I, Ibarra C. Water channel proteins AQP3 and AQP9 are present in syncytiotrophoblast of human term placenta. Placenta 2001; 22: 776-781
2. Damiano AE, Zotta E, Ibarra C. Functional and Molecular Expression of AQP9 Channel and UT-A Transporter in Normal and Preeclamptic Human Placentas. Placenta 2006; 27(11-12):1073-81.
3. Corominas AI, Balconi S, Palermo M, Maskin B, Damiano AE. Niveles de ácido úrico sérico y riesgo de desarrollar preeclampsia. Medicina 2014, 74(6):462-71.
4. Szpilbarg N, Castro-Parodi M, Reppetti J, Repetto M, Maskin B, Martinez N, Damiano AE. Placental programmed cell death: insights into the role of aquaporins. Mol Hum Reprod. 2016, 22(1):46-56.
5. Levi L; Castro-Parodi M; Martínez M; Piehl LL; Rubín de Celis E; Herlax V; Mate S; Farina M; Damiano AE. The unfavourable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas. Biochim Biophys Acta. 2016; 1858(9):2171-80.
6. Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor-1. Abán C, Martinez N, Carou C, Albamonte I, Toro A, Seyahian A, Franchi A, Leguizamón G, Trigubo D, Damiano A, Farina M. Apoptosis. 2016  21:1094-1105.
7. TPRV-1 expression in human preeclamptic placenta. Martínez N, Abán CE, Leguizamón GF, Damiano AE, Farina MG. Placenta. 2016 40:25-8.
8. Differential expression of endocannabinoid system in normal and preeclamptic placentas: Effects on nitric oxide synthesis. Abán C, Leguizamón GF, Cella M, Damiano A, Franchi AM, Farina MG. Placenta. 2013 34(1):67-74
9. Dual effect of anandamide on rat placenta nitric oxide synthesis. Cella M, Leguizamón GF, Sordelli M.S., Cervini M, Guadagnoli T, Ribeiro ML, Franchi A.M. and Farina M.G. Placenta 2008 29(8):699-707.
10. Sex-dependent changes in lipid metabolism, PPAR pathways and microRNAs that target PPARs in the fetal liver of rats with gestational diabetes. Fornes D, White V, Higa R, Heinecke F, Capobianco E, Jawerbaum A. Mol Cell Endocrinol. 2017 Aug 12 [Epub ahead of print]
11. Pro-oxidant/pro-inflammatory alterations in the offspring´s heart of mild diabetic rats are regulated by maternal treatments with a mitochondrial antioxidant. Higa R, Roberti SL, Capobianco E, Fornes D, White V, Jawerbaum A. Reprod Toxicol. 2017 Jun 23. [Epub ahead of print
12. Review on intrauterine programming: Consequences in rodent models of mild diabetes and mild fat overfeeding are not mild. Jawerbaum A, White V. Placenta. 2017 Apr;52:21-32.
13. A novel rat model of gestational diabetes induced by intrauterine programming is associated with alterations in placental signaling and fetal overgrowth. Capobianco E, Fornes D, Linenberg I, Powell TL, Jansson T, Jawerbaum A. Mol Cell Endocrinol. 2016 Feb 15;422:221-232.
14. Maternal saturated-fat-rich diet promotes leptin resistance in fetal liver lipid catabolism and programs lipid homeostasis impairments in the liver of rat offspring. Mazzucco MB, Fornes D, Capobianco E, Higa R, Jawerbaum A, White V. J Nutr Biochem. 2016 Jan;27:61-9.
15. Effects of maternal dietary olive oil on pathways involved in diabetic embryopathy. Higa R, Roberti SL, Musikant D, Mazzucco MB, White V, Jawerbaum A. Reprod Toxicol. 2014 Nov;49:185-95.
16. Intrauterine effects of impaired lipid homeostasis in pregnancy diseases. Higa R, Jawerbaum A. Curr Med Chem. 2013;20(18):2338-50. Review.
17. Review: Effects of PPAR activation in the placenta and the fetus: implications in maternal diabetes. Jawerbaum A, Capobianco E.Placenta. 2011 Mar;32 Suppl 2:S212-7.
18. Animal models in diabetes and pregnancy. Jawerbaum A, White V. Endocr Rev. 2010 Oct;31(5):680-701.